Fig. 2. Single-molecule imaging in the absence (A and E) and presence of M.SssI MTase (B and F), M.CviPI MTase (C and G) and M.SssI MTase + M.CviPI MTase (D and H), respectively. The Cy5 fluorescence signals are shown in red color, and the Cy3 fluorescence signals are shown in green color. The 100 U mL⁻¹ M.SssI MTase and 500 U mL⁻¹ M.CviPI MTase are used in the experiments. The scale bar is 2 μm.