Sequences of the oligonucleotides^a.

Note	Sequences (5′–3′)
HS1
HS2
SP1	AGC AT̲G GAG TCG ATT–SH
SP2	TGA T̲AG TAG AGT TTT–SH
CP1	GTG TTC TCG ACT CCA TGC TGA ACA C
CP2	GCA ATA ACT CTA CTA TCA ATT GC

^aIn HS1 and HS2, the underlined regions signify the recognition sites for GlaI, and the italicized regions signify the recognition sites for M.SssI and M.CviPI MTases, respectively. In HS2, the bold base “C” indicates the modified 5-mC. In SP1 and SP2, the underlined bases “T” indicate the modification with Cy5 and Cy3, respectively, and the italicized bases “G” indicate guanine ribonucleotides, and the “SH” indicates the modification of a sulfhydryl group.