Fig. 2. (A) Assigned 1H, 15N-HSQC spectra of the GTP-bound KRAS-WT (dark green), G12C mutant (blue) and G12D mutant (purple). The assignment of the newly detected and assigned crosspeaks of the KRAS-G12C mutant is shown (the full assignment is deposited in BMRB, access number: 28015). (B) Secondary structure propensities (SSP) of the GTP-bound KRAS-G12C (blue) compared with the GDP-bound form (cyan) along with the residue numbers. Secondary structure elements are drawn above the graph, and the P-loop (light red, residues 10–17), switch I (yellow, residues 25–40) and switch II (light blue, residues 57–76) units are depicted.
