Fig. 4. Selected dynamic properties of KRAS WT (light green/green), G12C mutant (cyan/blue), G12D mutant (lilac/purple) in GDP-bound (left) and GTP-bound forms (right): (A) generalized order parameter, S2, (B) correlation times for internal motions, τe, and (C) slow exchange rates, Rex, all of them derived from the Lipari–Szabo model-free formalism. (D) Spectral density values of fast timescale motions according to the reduced spectral density mapping method, J(ωh). P-loop (light red, residues 10–17), switch I (yellow, residues 25–40) and switch II (light blue, residues 57–76) are shown in colored rectangles (for individual values of the most interesting switch I residues see ESI Table 1†).
