Averaged relaxation parameters (R1, R2 and HetNOE) determined for KRAS-WT, G12C and G12D in both GDP- and GTP-bound forms. Additional dynamic properties were calculated with the Lipari–Szabo model-free and reduced spectral density analysis: τc (global correlation time), S2 (generalized order parameters) and J(ωh): the spectral density functions of fast motions.
| KRAS-WT | KRAS-G12C | KRAS-G12D | ||||
|---|---|---|---|---|---|---|
| +GDP | +GTP | +GDP | +GTP | +GDP | +GTP | |
| R 1 (s−1) | 0.97 ± 0.05 | 0.85 ± 0.08 | 0.99 ± 0.06 | 0.85 ± 0.07 | 0.95 ± 0.06 | 0.82 ± 0.07 |
| R 2 (s−1) | 13.85 ± 2.00 | 16.94 ± 3.12 | 14.16 ± 2.01 | 16.57 ± 2.63 | 14.83 ± 3.37 | 17.54 ± 2.76 |
| HetNOE | 0.79 ± 0.10 | 0.75 ± 0.14 | 0.79 ± 0.10 | 0.77 ± 0.13 | 0.78 ± 0.10 | 0.76 ± 0.13 |
| τ c (ns) | 9.95 | 12.33 | 9.97 | 11.97 | 10.44 | 12.59 |
| S 2 | 0.82 ± 0.07 | 0.85 ± 0.09 | 0.84 ± 0.08 | 0.84 ± 0.08 | 0.84 ± 0.07 | 0.85 ± 0.08 |
| J(ωh) (ps rad−1) | 3.26 ± 1.92 | 3.33 ± 2.29 | 3.30 ± 1.86 | 3.22 ± 2.34 | 3.35 ± 1.85 | 3.11 ± 1.94 |