. 2021 May 27;26(21):2000022. doi: 10.2807/1560-7917.ES.2021.26.21.2000022

Table 3. Summary of mutations found in the 14 strains of Klebsiella pneumoniae analysed, Marseille, France, 2014–2017 (n = 14 isolates).

Strain	ST	wzi	Colistin MIC (µg/mL)	PmrA	PmrB	PhoP	PhoQ	MgrB	CrrB	CrrA	AcrR	AcrS	mcr
KP1CR	307	NF^a	16	A41T [13]	L213M [13]; T246A [26,37]	No^b	No^b	No^b	C68S	No^b	No^b	S76R	No^b
KP2CR	15	NF^a	16	G53V^c [26]	No^b	No^b	No^b	No^b	NF^a	NF^a	No^b	No^b	No^b
KP3CR	307	NF^a	16	A41T	L213M; T246A	No^b	No^b	No^b	C68S	No^b	No^b	S76R	No^b
KP4CR	307	173	8	A41T	L213M; T246A	No^b	No^b	No^b	C68S	No^b	No^b	S76R	No^b
KP5CR	307	173	16	A41T	L213M; T246A	L12Q	No^b	No^b	C68S	No^b	No^b	S76R	No^b
KP6CR	15	NF^a	16	No^b	No^b	No^b	L87P	No^b	NF^a	NF^a	No^b	No^b	No^b
KP7CR	307	173	16	A41T	L213M; T246A	No^b	No^b	No^b	C68S	No^b	No^b	S76R	No^b
KP8CR	307	173	32	A41T	L213M; T246A	No^b	No^b	Stop (13AA) ^d	C68S	No^b	No^b	S76R	No^b
KP11CR	101	137	16	A217V	T246A	No^b	No^b	No^b	NF^a	NF	No^b	H79Q	No^b
KP12CR	307	173	8	A41T	L213M; T246A	No^b	No^b	No^b	C68S	No^b	No^b	S76R	No^b
KP13CR	307	173	16	A41T	L213M; T246A	No^b	No^b	No^b	C68S	No^b	No^b	S76R	No^b
KP14CR	307	173	4	A41T	L213M; T246A	No^b	No^b	No^b	C68S	No^b	No^b	S76R	No^b
KP15CR	322	50	> 64	S64T [26]	No^b	No^b	No^b	IS10 in bp 76 [38]	C68S	No^b	No^b	P211S	No^b
KP16CR	101	137	4	A217V [13]	T246A	No^b	N135K	No^b	NF^a	NF^a	No^b	H79Q	No^b

AA: amino acid; CR: colistin resistant; IS: Insertion Sequence; KP: Klebsiella pneumoniae; MIC: minimum inhibitory concentration; NF: not found^a; PROVEAN: Protein Variation Effect Analyzer; ST: sequence type.

^aThe gene was not found.

^b No mutation was found in the gene compared with the reference strain MGH78578.

^c A mutation at this position was previously reported to alter protein function, but the substitution was G53C.

^d Stop codon at the codon for the 13^th AA, leading to a truncated protein.

Mutations in bold were predicted to modify protein function by PROVEAN analysis. Studies that have previously reported some of the mutations listed in the Table are respectively cited next to the mutation.