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Rapamycin-induced ERK1/2 activation occurs downstream of p70S6K/PI3K. The effects of rapamycin (1000 nmol/L, 2 h) and/or PI3K inhibitor LY294002 (10 µmol/L, 2 h) on Akt(Thr308), ERK, and p70S6K phosphorylation in MCs (n = 3) were analyzed by western blotting.
&P < 0.05.
