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. 2021 May 28;29:100930. doi: 10.1016/j.mgene.2021.100930

Fig. 4.

Fig. 4

TMPRSS2 Expression. (A) Representative image showing higher mRNA levels of TMPRSS2 in variant carriers as compared to wild type (B)Representative IHC image (10×) showing higher TMPRSS2 protein expression in variant carriers (right panel) as compared to wild type (left panel). Arrows indicate the epithelial lining (C) Left Panel indicating Immunoblot analysis of HEK 293 T cells transfected with indicated constructs using Flag tag antibody. Membranes were stripped and re-probed with β-actin antibody to ensure uniform loading. *, non-specific and Right Panel depicts Densitometry quantification of unprocessed band (~54 kDa form) intensity using ImageJ. Data represents mean + SD of 4 independent experiments. Statistical analysis was performed by Student's ‘t’ test. #, p < 0·05. (D) Left panel representing Immunoblot analysis of HEK 293 T cells transfected with the indicated constructs using Flag tag antibody. Membranes were stripped and re-probed with GAPDH antibody to ensure uniform loading. Uncleaved, S2 and S2’ fragments highlighted. *, non-specific and Right Panel depicting Densitometry quantification of S2’ band intensity using ImageJ. Data represents mean + SD of 4 independent experiments. Statistical analysis was performed by Student's ‘t’ test. ##, p < 0·01.