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. 2021 May 27;21:281. doi: 10.1186/s12935-021-01968-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — hnRNPA2B1 regulated cancer stem–like cell features of GC. a Tumorsphere assays were performed to assess the cancer stemness of HGC-27 and MGC-803 cells. Upper, representative images are shown. Lower, statistical graphs. b Western blotting of hnRNPA2B1 and CSC markers, CD44 and Sox2 expression in HGC-27 and MGC-803 cells upon hnRNPA2B1 knockdown. c qRT-PCR was performed to evaluate the mRNA expression levels of CD44 and hnRNPA2B1 in monolayer GC cells and tumorspheres obtained from GC cells. d FACS analysis of CD44-positive cells by for tumorsphere cells after transfection with control shRNA or hnRNPA2B1 shRNA. The percentage was shown. e, f The results from HGC-27 cells (e) or MGC-803 cells (f) transfected with control shRNA or a shRNAs targeting hnRNPA2B1 and treated with cisplatin for 48 h. The IC50 value was determined by Prism 8 software. Data are presented as means ± S.D