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. 2021 May 28;21:242. doi: 10.1186/s12870-021-03033-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Anthocyanin accumulation in wild-type, single mutants of glk1 and glk2, and the glk1 glk2 double mutant. a Real-time quantitative PCR analysis of the AtGLK1 and the AtGLK2 transcript accumulation in the wild type (Col), single mutants of glk1 and glk2, and the glk1 glk2 double mutant seedlings. The total RNA was isolated from 4-d-old seedlings grown on 1/2 MS medium supplemented with 2% sucrose. b Images of representative seedlings of the wild-type (Col), single mutants of glk1 and glk2, and the glk1 glk2 double mutant grown for 4 days on 1/2 MS medium supplemented without sucrose (-S), with 2% sucrose (+ S), or with 58 mM mannitol (Man), respectively. The black arrows indicate the locations of the anthocyanin accumulation in different genotypic Arabidopsis seedlings. c Quantitative measurement of anthocyanins in 4-d-old seedlings (Col, glk1, glk2 and glk1 glk2) grown on 1/2 MS medium supplemented without sucrose (-S), with 2% sucrose (+ S), or with 58 mM mannitol (Man), respectively. The asterisks indicate statistically significant differences compared with the corresponding wild-type (Student’s t test: *P < 0.05)