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. 2021 May 28;21:242. doi: 10.1186/s12870-021-03033-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — AtGLK1 acts upstream of MYBL2 to genetically regulate anthocyanin accumulation in Arabidopsis. a Expression analysis of AtGLK1 and MYBL2 in the wild-type (Col), AtGLK1-overexpressing (OEGLK1), MYBL2-overexpressing (OEMYBL2), and 35S::MYBL2/OEGLK1 (OEGLK1/OEMYBL2) double overexpressing plants. b Images of representative seedlings and anthocyanin contents c of wild-type, AtGLK1-overexpressing, MYBL2-overexpressing, and 35S::MYBL2/OEGLK1 double overexpressing lines. d Transcript levels of structural (CHS, CHI, DFR, F3'H, LDOX, UF3GT, UGT75C1, and UGT75C2) and regulatory (PAP1 and TT8) genes involved in anthocyanin biosynthesis in wild-type, AtGLK1-overexpressing, MYBL2-overexpressing, and 35S::MYBL2/OEGLK1 double overexpressing plants. Total RNA extracted from the different genotypic seedlings grown on 1/2 MS medium for 4 days. The asterisks indicate statistically significant differences compared with the corresponding wild-type (Student’s t test: *P < 0.05)