Fig. 4. (a) Evaluation of 1O2 generation in HepG2 cells with DCFH-DA and SOSG. The scale bar represents 20 μm. (b) CLSM images of calcein AM/PI-stained HepG2 cells. The scale bar represents 20 μm. (c) In vivo fluorescence imaging of HepG2 tumor-bearing BALB/c mice after intravenous injection of BIBCl–PAE NPs (injected at a dose of 2 mg kg−1 BIBCl). (d) Normalized fluorescence intensity of the photosensitizer in tumor sites at different time points. (e) Fluorescence images of ex vivo organs harvested at 24 h post injection.
