Skip to main content
. Author manuscript; available in PMC: 2021 May 28.
Published in final edited form as: Nat Cell Biol. 2021 Apr 1;23(4):341–354. doi: 10.1038/s41556-021-00653-6

Extended Data Fig. 4. EZH2 alters box C/D snoRNP assembly.

Extended Data Fig. 4

(a) Mixtures of recombinant EZH2, FBL and NOP56 proteins with or without BS3 crosslinking were subjected to SDS-PAGE, followed by western blot analysis using their own antibodies to visualize the location of cross-linked species.

(b) Gel band containing cross-linked proteins was subjected to Mass Spectrometry analyses to detect the presence of all three proteins.

(c-f) Densitometric analysis of data from Fig. 4a were shown as distribution proportion of FBL (c)/NOP56 (d)/NOP58 (e)/SNU13 (f) protein in each fraction.

(g) Fractions 17–19 and 27–29 from control C4–2 cell nuclear extracts were subjected to co-IP assay using anti-EZH2 antibody.

The assays in a-b and g have been performed three times with similar results. Statistical source data and unprocessed blots are provided in Source data Extended data Fig. 4.