Skip to main content
. 2021 May 14;11:672687. doi: 10.3389/fonc.2021.672687

Figure 1.

Figure 1

CSV-CTCs are correlated with LC patients ’ cancer stages, lymph node and distant metastases and can be used to distinguish LC patients from patients with BLD and HD. (A) Immunofluorescent staining of a captured CSV-CTC and a white blood cell (WBC), indicated by the yellow and white arrows, respectively. CSV positive CTCs are defined as DAPI (blue) positive, CSV (FITC, green) positive and CD45 (PE, orange) negative cells, while a WBC as a DAPI positive, CD45 positive and CSV negative cell. Scale bar represents 10 μm, immunofluorescent staining, X 20 (B) CSV-CTC enumeration can differentiate LC patients from BLD patients and HD (both P < 0.0001). (C) CSV-CTCs are correlated with cancer stage (P = 0.0062). Significant differences of CSV-CTCs are found between BLD patients and stage I, II, III or IV LC patients (P = 0.0167, 0.0307, 0.0014, or < 0.0001). CSV-CTCs are correlated as well with age (P = 0.0274), lymph node metastasis (P = 0.0002) and distant metastasis (P = 0.0021), as shown in (D, F, G), respectively. However, CSV-CTCs are not associated with tumor depth as shown in (E). “*”, “**”, “***”, and “****” indicates 0.01 < P < 0.05, 0.001 < P < 0.01, 0.0001 < P < 0.001, and P < 0.0001, respectively.