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. Author manuscript; available in PMC: 2022 May 18.
Published in final edited form as: Lab Chip. 2021 May 18;21(10):1956–1973. doi: 10.1039/d0lc01323d

Fig. 1. Fetal and maternal interface (FMi) recreation using OOC device.

Fig. 1.

(a) An illustration of fetal maternal interface (FMi) tissue in utero anatomy (black box). FMi consists of several different cell types (zoom out box), fetal derived cells: amnion epithelial cells (AECs), the most closest cells to fetus; amnion mesenchymal cells (AMCs); chorion mesenchymal cells (CMCs); chorion trophoblast cells (CTCs); maternal derived cells: decidua cells (DEC). (b) FMi in Histology. Close-up images for each cell type displayed. Scale bar, 100 μm. (c) Schematic illustration of the FMi-OOC highlighting the four concentric cell culture chambers and block reservoir filled with color dye in each of the corresponding cell culture layers. Close up schematic illustration of the cells within the FMi-OOC separated by arrays of 24 microchannels. (d) Representative histological image comparing the structure of FMi tissue to FMi-OOC device. Scale bar, 100 μm. (e) Cross-sectional view of the cell culture chambers showing the chamber height (250 μm), individual chamber diameters (mimicking in utero thickness), and the interconnecting microchannel lengths. (f) Image of the microfabricated FMi-OOC where each cell culture chamber was filled with different color dye for easy visualization. AECs (chamber 1), AMCs (chamber 2), CTCs (chamber 3) and DEC (chamber 4) were seeded for exosome trafficking study.