Putative DNA adducts that displayed changes in the DNA of NMC-exposed B. subtilis. Putative names are reported, as structural identification can only be confirmed by comparison with synthetic standards in future work. [M + H]⁺ values marked with bold font indicate overlapping [M + H]⁺ with S. oneidensis.

[M + H]+	Fold change	Putative name	Origin	Category
229.0931	62	dIZ	Degradation of 8-oxo-dG	Oxidative stress
244.0928	519	5-OH-dC	Direct or Indirect Oxidation via ROS	Oxidative stress
260.0877	15 234	5,6-OH-dC	Direct or Indirect Oxidation via ROS	Oxidative stress
262.1034	4581	5,6-H-5,6-OH-dC	Direct or Indirect Oxidation via ROS	Oxidative stress
284.0989	204	8-oxo-dG	Direct or Indirect Oxidation via ROS	Oxidative stress
286.1146	Only in exposed	FAPY-dG	Direct or Indirect Oxidation via ROS	Oxidative stress
297.0942	Only in exposed	Nitro-dA	Direct or Indirect Oxidation via ROS	Oxidative stress
310.1146	Only in exposed	N 6 -hydroxyacetyl-dA	Direct or Indirect Oxidation via ROS	Oxidative stress
252.0979	Only in exposed	3,N2-εdC	Lipid peroxidation	Lipid peroxidation
382.1721	Only in exposed	N 2 -dimethyldioxane-dG	Lipid peroxidation	Lipid peroxidation
306.1197	Only in exposed	M1dA	Malonaldehyde	Lipid peroxidation
386.1459	Only in exposed	M2AA-dA	Malonaldehyde	Lipid peroxidation
402.1408	Only in exposed	M2AA-dG_I	Malonaldehyde	Lipid peroxidation
338.1459	Only in exposed	CRO-dG	Crotonaldehyde	Lipid peroxidation
299.1238	Only in exposed	Acr-dT_I	Acrolein	Lipid peroxidation
308.1353	Only in exposed	Acr-dA_I	Acrolein	Lipid peroxidation
591.2270	943	dG-ACR-dG	Acrolein	Lipid peroxidation
300.1190	18	Carboxy-dC	Glycation	Glycation
324.1302	52/Only in exposeda	Carboxy-dA	Glycation	Glycation
370.1357	Only in exposed	CHPG-dG	Glycation	Glycation
266.1248	7	N 6-methyl-dA	Alkylating agent	Alkylation
296.1353	27	N 2-ethyl-dG	Alkylating agent	Alkylation

^aTwo isomers were found for this m/z at different retention times.