Fig. 3. (a) One-photon excitation (OPE) and two-photon excitation (TPE) imaging of HeLa cells pretreated with EBD-1 and EBD-5. (b) EBD-1 pre-treated HeLa cells co-localized with CellMask Green without irradiation. Red channel for EBD-1 (λex = 488 nm, λem = 630 nm) and the green channel for CellMask Green (λex = 522 nm, λem = 535 nm). (c) The co-staining experiment between EBD-1 and DAPI with irradiation for 20 min: red channel for EBD-1 and the blue channel for DAPI (λex = 405 nm, λem = 420–450 nm). (d) EBD-5 pretreated HeLa cells co-localized with CellMask Green without irradiation. Red channel for EBD-5 (λex = 488 nm, λem = 630 nm) and the green channel for CellMask Green. (e) The co-staining experiment between EBD-5 and MitoTracker Deep Red with irradiation for 20 min. Green channel for EBD-5 and the red channel for MitoTracker Deep Red (λex = 640 nm, λem = 659 nm) (808 nm laser; 200 mW cm−2). Scale bar: 20 μm.
