Fig. 4. (a) Confocal images of three cancer cells (HeLa, A431, and Hep G2) and four normal cells (3T3, HEK 293T, HFL-1 and HELF), incubated with EBD-1 (10⁻⁵ M) for 30 min. (b) Fluorescence intensity of different cell lines stained using EBD-1. (c) Viabilities of different cell lines treated with EBD-1 (10⁻⁵ M) under different conditions for 10 min (dark or 808 nm laser; 200 mW cm⁻²). (d) Fluorescence intensity distribution of HeLa (yellow circle) and HELF cells (white circle) cultivated in the same dish. (e) Fluorescence intensity of the two cell lines stained with EBD-1. All of the fluorescence intensities of different cell lines were obtained using cell image processing software ImageJ. Scale bar = 20 μm.