Comparison of the kinetic constants of SAzymes with those of natural enzymes.

Sample	[E] (M)	Substrate	K m (mM)	V m (μM s−1)	k cat (s−1)	k cat/Km (mM−1 s−1)	Ref.
PMCSa	2.147 × 10−14	TMB	0.224	0.1066	5.66 × 106	2.5268 × 107	66
		H2O2	40.160	0.1215	4.97 × 106	1.2376 × 105
Fe–N-rGO sheetsa	1.2 × 10−11	TMB	0.074	1.74	1.45 × 105	1.9595 × 106	85
		H2O2	43	1.44	1.20 × 105	2.7907 × 103
Fe–N–C SANa	1.6 × 10−11	TMB	0.08	0.7452	4.66 × 104	5.8219 × 105	67
		H2O2	28.3	0.4285	2.68 × 104	9.4633 × 102
CNT/FeNCb	1.7 × 10−7	TMB	0.1294	0.156	0.9176	7.0912	87
		H2O2	—	—	—	—
SAF NCsb	6.07 × 10−6	TMB	—	—	—	—	68
		H2O2	0.0120	0.223	0.0367	3.0711
Fe SAEsb	1.07 × 10−6	TMB	3.92	0.588	0.5495	0.1402	78
		H2O2	0.243	0.0825	0.0771	0.3173
Fe–N–Cb	1.48 × 10−6	TMB	3.6	1.16	0.7838	0.2177	103
		H2O2	12.2	0.356	0.2405	0.0197
Cu–N–Cb	2.68 × 10−6	TMB	3.76	0.7505	0.2803	0.0746	113
		H2O2	19.94	0.2007	0.0750	0.0038
Fe–N–C SAzymesb	—	TMB	5.20	1.49	—	—	65
		H2O2	4.31	0.62	—	—
Co–N–C SAzymesb	—	TMB	5.06	0.190	—	—
		H2O2	16.26	0.165	—	—
Zn–N–C SAzymesb	—	TMB	0.28	0.043	—	—
		H2O2	6.27	0.048	—	—
HRP	2.5 × 10−11	TMB	0.434	0.100	4.00 × 103	9.2166 × 103	17
		H2O2	3.7	0.087	3.50 × 103	9.4595 × 102

^a[E] is the concentration of nanozymes.

^b[E] is the concentration of the metal in nanozymes.