Fig. 4. Evaluation of the compatibility of phospholipid-based vesicles and activation chemistry. (A) Plot reporting the yield (%) of nucleotide (A3′P) activation in the absence and presence of vesicles composed of MDG2P after 24 h in DCI buffer, pH 6. (B) Schematic representation of the two potential reaction pathways leading to the activation of both lipids and nucleotides. The preferential pathway describes the selectivity of lipid activation, followed by nucleotide activation. (C) Plot representing the yield (%) of nucleotide (A3′P) activation in the presence of phospholipid-based vesicles in DCI buffer, pH 6. The profile of nucleotide activation in the absence of vesicles (dark pink line) is reported for comparison. Plotted data are representative of ³¹P-NMR experiments.