Fig. 3. (a) Mass spectra of BSA before (red) and after (blue) reduction with DTT for 1 h. (b) Mass spectrum of BSA after high-temperature hydrolysis without reduction. (c) Mass spectrum of BSA treated by separated procedures: reduction by incubation with DTT for 1 h and then high-temperature hydrolysis for 5 min. (d) Mass spectrum of BSA by one-step high-temperature hydrolysis and reduction for 5 min. Peptides identified by MASCOT were marked with * and listed in Tables S2–S4.†.
