Fig. 6. Analysis of ESAT-6 by high-temperature hydrolysis and a miniature MS system. (a) Schematic of the miniature MS system. Mass spectra of ESAT-6 (b) before and (c) after high-temperature hydrolysis, showing fast and sensitive determination of the characteristic peptide highly specific to Mtb. ESAT-6 was prepared in water (2% formic acid, v/v) at the concentration of 20 ppm. The solution after treatment with the high-temperature microreaction system (150 °C, 2 min) was directly used for nanoESI-MS analysis.
