Fig. 3. Fetal bovine serum (FBS) and RNase A stability of native siRNA and caged Multi-Chol-siRNA self-assembled nanoparticles. The native siRNA and caged Multi-Chol-siRNA self-assembled nanoparticles were incubated with 20% FBS solution at 37 °C for predetermined time intervals (A) and with 0–1 mg L−1 RNase A (B). Percentages of siRNA were evaluated by normalizing the relative intensity of each band over the control band on the same gel using Image J software. Each sample was irradiated to release siRNA for gel electrophoresis. Data are presented as mean ± S.E.M. (n = 3).
