Fig. 5. Photoregulation of Eg5 gene silencing with the caged Multi-Chol-siRNA self-assembled nanoparticles. (A) Nuclei phenotypes of HepG2 cells using a confocal laser scanning microscope. Scale bar = 20 μm. (B) Percentage of cells in G2/M phases of the cell cycle quantified via a cycle analysis kit. (C) Photoregulation of Eg5 mRNA levels determined by real-time qPCR. The concentration of PC (AE/SE) is 5 nM. Data are presented as mean ± S.E.M. (n = 3).
