Table 2.

qPCR Primer Sequences

Gene Name (Abbreviation)	Forward Sequence
Glyceraldehyde 3-phosphate dehydrogenase (gapdh)	F: 5′—ACCCCTTCATCGACTTGGAC—3′ R: 5′—GGAGCCAGACAGTTTGTAGTG—3′
Krüppel-like factor 9 (klf9)	F: 5′—GTGGCCACTTGATTTCCCCT—3′ R: 5′—AAAGACACAAAACAGCGGCG—3′
Thyroid receptor β (trβ)	F: 5′—CTCATAGAAGAAAACAGAGAAAARAGA—3′ R: 5′—GAAGGCTTCTAAGTCCACTTTTCC
Deiodinase 3 (dio3)	F: 5′—GATGCTGTGGCTGCTGGAT—3′ R: 5′—ATTCGGTTGGAGTCGGACAC—3′
Thyroid-stimulating hormone β (tshβ)	F: 5′—ACTGCATGACAAAGGATCCAAA—3′ R: 5′—CAGGGTAGGAAAAGAGCGGG—3′
Thryoid-releasing hormone (trh)	F: 5′—ACGTGAAGATCTCAAGAAGGT T—3′ R: 5′—CACCAGAACTGTGTGGCAAG—3′
Corticotropin-releasing hormone (crh)	F: 5′—CCCAGCCCTTTTTACTGCGA—3′ R: 5′—AGATGCCTCAGGAAACGAGC—3′

Note: F: forward primers; R: reverse primers.