Fig 8. M2-1 binds specifically to host cell RNA.

(A) CLIPper-identified peaks were graphed for fold enrichment vs significance using ggplot2. Peaks within the region identified in pink were considered significant based on eCLIP standards. All peaks were plotted from each sample for 8 and 18 hpi for RSV infected (black) and mock infected (blue). (B) Gene identities of significant peaks were determined and common genes between samples were identified. (C) RNA immunoprecipitation followed by RT-qPCR of target genes identified by CLIPper. Levels of co-immunoprecipitated RNA were normalized to levels of co-immunoprecipitated N mRNA. Individual data points for two biological replicate experiments are shown.