Fig. 2.

a Radiochromato-electrophoretograms of antiserum and insulin mixtures. Mixtures contained the same concentrations of guinea pig antibeef insulin serum and beef insulin-I131 but varying concentrations of human insulin as indicated. Adapted with permission from Yalow et al. [22]. b Ratio of bound insulin-I131 to free insulin-I131 as a function of concentration of added human insulin. The ratios were obtained from the complete series of radiochromato-electrophoretograms as shown in a. Adapted with permission from Yalow et al. [22].