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. 2020 Oct 9;11(42):11366–11379. doi: 10.1039/d0sc04568c

The photophysical properties of probes 1–34.

Probe Solvent Max λex/nm Max λem/nm ε a/M−1 cm−1 Φ b K d c LODd
No Zn2+ With Zn2+ No Zn2+ With Zn2+ No Zn2+ With Zn2+ No Zn2+ With Zn2+
1 HEPES buffer 470 470 517 517 NRe NR <0.005 0.18 126 nM NR
2 PIPES buffer 643 637 663 662 3.68 × 104 3.62 × 104 0.038 0.21 4 pM NR
3 HEPES buffer 493 493 515 515 7.3 × 104 NR 0.0032 0.225 0.45 μM NR
4 HEPES buffer 347 347 420 443 NR NR NR NR NR NR
5 MOPS/DPPC (1 : 1) 400 415 506 520 1.64 × 104 NR 0.19 NR 20 ± 0.4 nM NR
6 MOPS buffer 545 NR 575 575 NR NR NR NR 65 ± 10 nM NR
7 PIPES buffer 497 490 522 517 3.3 × 104 4.2 × 104 0.36 0.80 1.5 ± 0.2 μM NR
8 PIPES buffer 569 573 629 631 2.84× 104 3.67 × 104 0.0355 0.5025 3.25 ± 0.12 nM NR
9 MOPS buffer 388 375 500 493 1.87 × 104 2.31 × 104 0.15 0.92 3.1 nM NR
10 HEPES buffer 413 395 536 536 1.85 × 104 2.10 × 104 0.0048 0.33 1.4 nM NR
11 MOPS buffer 392 NR 559 NR 1.32 × 104 NR 0.023 NR 17 ± 2 nM NR
12 HEPES buffer 453 391 625f 542f 6.2 × 103 4.2 × 103 NR 0.03 8.2 ± 0.2 nM NR
13 HEPES buffer 420 370 550 504 NR NR 0.11 0.22 0.45 ± 0.01 nM NR
14 CH3CN 347g 363g 633 633 81 × 103g 67 × 103g 0.28i 0.29j 9.1 μM NR
602h 602h 23 × 103h 21 × 103h
15 PIPES buffer 510 510 535 529 NR NR <0.001 0.75 ± 0.03 0.6 ± 0.03 nM NR
16 HEPES buffer 496 469 550 534 2.10 × 104 1.40 × 104 0.003 0.046 1.0 ± 0.1 nM NR
17 k MES buffer 285 261 542 495 4.30 × 103 1.66 × 103 0.11 0.17 16 ± 1.1 nM NR
18 l MES buffer 435 444 528 536 1.55 × 104 1.81 × 104 0.03 0.23 8.5 μM 0.18 μM
19 EtOH/Tris–HCl (9 : 1) 597 568 647 578 NR NR NR 0.23 68 ± 4 μMm 0.48 μM
123 ± 6 μMn
20 MES buffero 500 490 536 536 NR NR NR NR NR 0.477 μM
21a HEPES buffer/CH3CN (1 : 1) 350 365 504 504 NR NR 0.25 0.24 1 nM2 NR
21b 350 359 504 504 NR NR 0.32 0.33 0.17 nM2 NR
22a HEPES buffer 456 454 515 515 NR NR 0.0027 0.0482 1.54 nM NR
22b HEPES buffer 454 451 509 515 NR NR 0.0064 0.2606 2.57 nM NR
22c HEPES buffer 453 453 512 517 NR NR 0.0077 0.3283 1.91 nM NR
23a PIPES buffer 478 530 625 628 1.93 × 104 2.64 × 104 0.067 0.41 0.69 nM NR
23b PIPES buffer 480 524 630 630 1.69 × 104 2.56 × 104 0.069 0.22 0.70 nM NR
23c PIPES buffer 480 535 623 628 1.33 × 104 1.93 × 104 0.342 0.60 <0.001 nM NR
24 EtOH 406 NR 490 NR NR NR <0.01 0.12 5.5 nM 5.8 nM
25 HEPES buffer 346 346 414 414 NR NR 0.041 0.25 3.5 nM 47 pM
26 HEPES buffer 346 346 414 414 NR NR 0.013 0.041 4.7 nM 0.71 nM
27 HEPES buffer 346 346 414 414 NR NR 0.05 0.28 2.83 ± 0.11 nM 48 pM
28 PIPES buffer 515 507 530 525 7.95 × 104 8.40 × 104 0.39 0.87 0.7 ± 0.1 nM NR
29a PIPES buffer 499 496 519 514 2.72 × 104 1.60 × 104 0.004 0.73 p NR
29b PIPES buffer 498 492 515 515 6.41 × 104 4.00 × 104 0.012 0.51 q NR
30 HEPES buffer 302 360 532 532 420 4680 0.015 0.055 1.8 ± 0.1 pM 0.042 pM
32a MOPS buffer 384 388 518 518 2.89 × 104 NR 0.12l 0.93l 1.7 nMr NR
33 HEPES buffer 453 442 546 536 NR NR NR 0.19 4 nM 57 nM
34 HEPES buffer 346 346 414 414 NR NR 0.02 0.05 18.8 nM NR
a

Molar extinction coefficient.

b

Fluorescence quantum yield.

c

Dissociation constant.

d

The limit of detection.

e

Not reported.

f

λ ex = 466 nm.

g

The parameters of donor.

h

The parameters of acceptor.

i

λ ex = 565 nm.

j

λ ex = 400 nm.

k

All results shown here were recorded at lysosomal pH 5.2.

l

All results shown here were recorded at lysosomal pH 5.0.

m

Calculated from turn on fluorescence emission at wavelength 578 nm.

n

Calculated from the ratio of F578 nm/F680 nm.

o

The pH of the buffer is 5.0.

p

K d1 = 3.5 ± 0.1 mM, Kd2 = 150 ± 100 μM.

q

K d1 = 220 ± 30 μM, Kd2 = 160 ± 80 μM, Kd3 = 9 ± 6 μM.

r

The results were obtained in solution EtOH : MOPS buffer (1 : 1, v/v).