2.12.
Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-mouse MFN2 | Abcam | ab56889 RRID: AB_2142629 |
| Anti-mouse actin | Sigma | A2228 RRID: AB_476697 |
| IRDye 800CW Donkey anti-Mouse | LI-COR Biosciences | 926-32212 RRID: AB_2716622 |
| Anti-rabbit | LI-COR Biosciences | 926-32211 RRID: AB_621843 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| MEM alpha medium | Gibco Life Technologies | A1049001 |
| β-glycerolphosphate disodium salt | Sigma | G9422 |
| L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate | Sigma | A8960 |
| RNA to cDNA EcoDry™ Premix | Takara Bio | 639549 |
| iTaq Universal SYBER Green Supermix | Bio-Rad | 1725121 |
| Halt™ Protease and Phosphatase Inhibitor Cocktail (100X) | ThermoFisher Scientific | 78442 |
| Nitrocellulose | Bio-Rad | 1620115 |
| 10% neutral buffered formalin | Di Russcio and Associates, Inc. | N/A |
| Calcein | Sigma | C0875 |
| Alizarin-30methyliminodiacetic acid | Sigma | A3882 |
| MitotrackerGreenFM | ThermoFisher Scientific | M7514 |
| LysotrackerRedDND-99 | ThermoFisher Scientific | L7528 |
| (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylicacid | Trolox; MilliporeSigma | 238813 |
| Critical Commercial Assays | ||
| NucleoSpin RNA kit | Machery-Nagel | 740955.5 |
| DC Protein Assay | Bio-Rad | 5000116 |
| RatLaps P1NP assay | Immunodiagnostic Systems | AC-33F1 |
| CTX-1 EIA assay | Immunodiagnostic Systems | AC-06F1 |
| L-Lactate assay kit | Eon Biosciences | 120001100A |
| Resazurin Sodium Salt | Sigma | R7017 |
| Oligonucleotides | ||
| MFN2 F- 5’-AAGCACTTTGTCACTGCCAAG-3’ R- 5’-TTGTCCCAGAGCATGGCATTG-3’ |
Integrated DNA Technologies | N/A |
| MFN1 F-5’-TTGGCAGGACAAGTAGTGGC-3’ R-5’-AGCAGTTGGTTGTGTGACC-3’ |
||
| ND1 (for mitochondrial DNA) F- 5’-CCC ATT CGC GTT ATT CTT-3’ R-5’-AAG TTG ATC GTA ACG GAA GC-3’ |
Integrated DNA Technologies | N/A |
| LPL(for genomic DNA) F- 5’-GGA TGG ACG GTA AGA GTG ATT C-3’ R- 5’-ATC CAA GGG TAG CAG ACA GGT-3’ |
Integrated DNA Technologies | N/A |
| PPIA/Cyclophillin A gDNA F- 5’-AGCATACAGGTCCTGGCATC-3’ R- 5’-TTCACCTTCCCAAAGACCAC-3’ |
Integrated DNA Technologies | N/A |
| RUNX2 F- 5’-AACGATCTGAGATTTGTGGGC -3’ R- 5’-CCTGCGTGGGATTTCTTGGTT -3’ |
Integrated DNA Technologies | N/A |
| OSX F- 5’-ATGGCGTCCTCTCTGCTTG -3’ R- 5’-TGAAAGGTCAGCGTATGGCTT -3’ |
Integrated DNA Technologies | N/A |
| COL1A1 F- 5’-AGACATGTTCAGCTTTGTGGAC-3’ R- 5’-GCAGCTGACTTCAGGGATG-3’ |
Integrated DNA Technologies | N/A |
| ALP F- 5’-CCAACTCTTTTGTGCCAGAGA -3’ R- 5’-GGCTACATTGGTGTTGAGCTTTT -3’ |
Integrated DNA Technologies | N/A |
| OPN F- 5’-AGCAAGAAACTCTTCCAAGCAA -3’ R- 5’-GTGAGATTCGTCAGATTCATCCG -3’ |
Integrated DNA Technologies | N/A |
| Cyclophilin F- 5’-AGCATACAGGTCCTGGCATC-3’ R- 5’-TTCACCTTCCCAAAGACCAC-3’ |
Integrated DNA Technologies | N/A |
| Osteocalcin: QT00259406 1 Mm_Bglap_1_SG QuantiTect Primer Assay | QIAGEN LTD | Cat#QT00259406 |