Fig. 2. (a) Inhibition activity of complexes 2a–2h and reference EGFR inhibitor 1b (10 μM) against EGFR (10 μg mL−1) as measured by a fluorescence-based kinase assay. The fluorescence intensity was detected at ex/em = 330 nm/450 nm. (b) Absorbance spectra of complexes 2a–2h (5 μM) in ACN. (c) Excitation and emission spectra of complexes 2a–2h (5 μM) in DMSO. (d) Emission decay curves of complexes 2a–2h (5 μM) in PBS buffer containing 0.5% DMSO. (e) Quantum yields of complexes 2a–2h (5 μM). (f) Photographs of complexes 2a–2h (5 μM) observed under UV light (365 nm) in dimethyl sulfoxide (DMSO) and PBS buffer containing 0.5% DMSO, respectively.
