Fig. 4. (a) Effects of complex 2f on the thermal stability of EGFR in cell lysates. A431 cells lysates were treated with 2f (1 μM) for 1 h. Time-resolved spectra of complex 2f (10 μM) in the presence of fluorescent organic dyes (b) thioflavin S (THS) and (c) coumarin 460 (Cm460) as two model matrix interferences to simulate the autofluorescence of biological samples in PBS buffer containing 1% DMSO. (d) Co-localization studies of complex 2f (1 μM, 30 min) with EGFR protein in A431 cells. The Pearson's correlation coefficient of complex 2f with EGFR protein was calculated to 0.950. ex/em = 405 nm/500–700 nm. Scale bar = 5 μm.
