Fig. 5. (a) Confocal imaging of EGFR knockdown A431 cells. EGFR knockdown A431 cells were incubated with 2f (1 μM) for 2 h at 37 °C. ex/em = 405 nm/500–700 nm. Scale bar = 50 μm. (b) Confocal imaging of normal LO2 cells and EGFR-overexpressing A431 cells. LO2 cells and A431 cells were incubated with 2f (1 μM) for 2 h at 37 °C. ex/em = 405 nm/500–700 nm. Scale bar = 50 μm.