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. Author manuscript; available in PMC: 2021 May 28.
Published in final edited form as: Science. 2020 Nov 20;370(6519):eabc8301. doi: 10.1126/science.abc8301

Figure 2. Improved sequence sampling reverses Hadzipasic et al.’s ancestral reconstructions.

Figure 2.

(A) The established phylogeny of the major eukaryotic groups. Polytomy, branching order not established. (B) Maximum likelihood phylogeny when AURK and PLK are densely sampled. Numbers in parentheses indicate the number of sequences in each group. Node labels, reconstructed ancestral sequences. Fungi and Holozoa are pink and cyan, respectively. Branch label with arrow, approximate likelihood ratio statistic for Fungi+Holozoa (p < 0.01 by χ2 approximation, ref. 13). (C) ML phylogeny given the constraint in panel A. (D) ML phylogeny given the constraint in A, except Fungi are constrained to split first. (E) Most states that confer allostery in Hadzipasic et al. are not conserved in extant AURKs that are allosterically regulated by TPX2. Green and orange, allosteric and non-allosteric states from Hadzipasic et al.; gray, other states. Site numbers are in panel H. (F, G, H) Reconstructed sequences on the phylogenies in panels B, C, and D, respectively. The maximum a posteriori states at the 15 sites that experimentally confer allostery/nonallostery are shown, colored as in panel E and shaded by their posterior probability. Row labels correspond to ancestral nodes in panels B–D.