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. Author manuscript; available in PMC: 2021 May 28.
Published in final edited form as: Cell Rep. 2021 Mar 30;34(13):108754. doi: 10.1016/j.celrep.2021.108754

Figure 1. A database of human neocortical cell subclass-specific accessible chromatin elements.

Figure 1.

(A) Workflow for human neocortical open chromatin characterization. See STAR Methods for details.

(B–D) High-quality nuclei (2,858 from 14 specimens) visualized by t-distributed stochastic neighbor embedding (t-SNE) and colored according to mapped transcriptomic cell types grouped into cell type subclass (B), sort strategy (C), or specimen (D).

(E) Transcriptomic abundances of 11 cell subclass-enriched marker genes (median counts per million [CPM] within subclass) for 11 subclasses of cell types identified in human MTG (Hodge et al., 2019).

(F) Eleven example subclass-specific marker genes demonstrating uniquely accessible chromatin elements in their vicinity (less than 50 kb distance to gene). Pileup heights are scaled proportionally to read number, and yellow bars highlight subclass-specific peaks for visualization. Dashed lines, introns; thick bars, exons; arrows, direction to gene body.

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