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. Author manuscript; available in PMC: 2021 May 28.
Published in final edited form as: Cell Rep. 2021 Mar 30;34(13):108754. doi: 10.1016/j.celrep.2021.108754

Figure 6. Enhancer-AAV testing in NHP ex vivo neocortical slices.

Figure 6.

(A) Workflow for acquiring fresh NHP neocortical tissue for AAV vector testing ex vivo.

(B–D) Transduction of ex vivo NHP neocortical tissue with various AAV2/PHP.eB enhancer-reporter vectors, resulting in diverse expression patterns. eHGT_078m labels excitatory neurons throughout all layers (B), eHGT_058h labels excitatory neurons primarily in L3–L5 (C), and DLX2.0 labels inhibitory neurons (D).

(E–K) NHP neocortical cell subclass specificity of AAV-vector labeling confirmed by mFISH. eHGT_078m, 058h, and DLX2.0 demonstrate high specificity similar to that seen in mouse retro-orbital assay, but eHGT_079h, 082h, 128h, and 140h show reduced specificity compared to that seen in mouse retro-orbital assay and NHP in vivo assay. Arrows highlight specifically labeled on-target cell types, and asterisks mark off-target labeled cells. Text represents mean for labeling specificity across one or two independent transduction experiments (>100 cells counted per vector per experiment).

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