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. 2021 Apr 8;72(12):4319–4332. doi: 10.1093/jxb/erab156

Fig. 2.

Fig. 2.

MYB21 and its homologs mediate flavonol biosynthesis through the regulation of flavonol synthase gene expression. (A) In situ flavonol staining of wild-type (Col-0) and myb21 inflorescences. Flavonols in ethanol-bleached inflorescences were stained with DPBA to saturation and imaged by epifluorescence microscopy. Bars=1 mm (for inflorescences) or 500 μm (for buds, pistils, and stamens). (B) Gene expression of FLS1, MYB21, CHS, CHI, F3H, and F3′H in inflorescences of wild-type (Col-0) and Pro35S:MYB21-FLAG (MYB21OE1–3) plants. Transcripts were analyzed by qRT–PCR. Error bars indicate the SD of three biological replicates. (C) Flavonol glycoside accumulation patterns of the inflorescences of wild-type and Pro35S:MYB21-FLAG (MYB21OE1–3) plants. Methanolic extracts were separated by HPTLC followed by DPBA staining and UV illumination. (D) Quantification of flavonols in the inflorescences of wild-type and Pro35S:MYB21-FLAG (MYB21OE1–3) plants. Error bars indicate the SD of three biological replicates. Different letters above the bars indicate significant differences in flavonol content between the wild type and other genotypes (P<0.05; ANOVA with Fisher’s LSD test).