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. 2021 May 28;7(22):eabg7653. doi: 10.1126/sciadv.abg7653

Fig. 6. α-Actinin-2 stabilizes FATZ proteins at the Z-disk.

Fig. 6

(A) COS-1 cells coexpressing enhanced CFP (ECFP)–tagged FATZ-1 (WT FATZ-1) or corresponding fiveE mutant, in combination with actinin-2-EYFP, as determined in fixed cells by acceptor photobleaching. Representative images are shown before and after bleaching for both proteins. Bleached regions of interest are shown (insets), along with FRET efficiencies used to calculate FRET ratios presented in the adjacent graph [n = 28 (WT) and 30 (fiveE), ****P < 0.0005, Student’s t test). (B) Same cells as in (A) but coexpressing ECFP-tagged FATZ-2 variants in combination with EYFP-α-actinin-2 [n = 26 (WT) and 25 (fiveE), ****P < 0.0005, Student’s t test). Scale bars, 10 μm in all images. See also fig. S17.