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. 2021 May 28;7(22):eabg3777. doi: 10.1126/sciadv.abg3777

Fig. 8. TDNs show distinct membrane excitability and synaptic activity and respond to physiological stimuli.

Fig. 8

(A) Representative voltage traces recorded from control and TDNs from young and adult mice evoked by depolarizing current steps as indicated. (B) The current-spike frequency relationships measured from control and TDNs from young (top) and adult mice (bottom). The current-frequency relationship was significantly different between TDNs and control neurons at both ages [young control neurons, 14 cells from four mice; young TDNs, 13 cells from four mice; P < 0.0001, two-way analysis of variance (ANOVA); adult control neurons, 16 cells from six mice; adult TDNs, 18 cells from six mice; P = 0.0001, two-way ANOVA]. (C) Representative traces of spontaneous postsynaptic currents (sPSCs). (D) Summary graphs of sPSC frequency (young control neurons, 14 cells from four mice, 2.85 ± 0.74 Hz; young TDNs, 13 cells from four mice, 4.07 ± 1.93 Hz; P = 0.2983, Mann-Whitney U test; adult control neurons, 16 cells from six mice, 7.56 ± 1.82 Hz; adult TDNs, 18 cells from six mice, 2.98 ± 0.54 Hz; P = 0.0324, Mann-Whitney U test). (E) Positive correlation between the distance from the tanycytic layer for each TDN and its sPSC frequency in both young (left: 12 cells from four mice, P = 0.0234, Spearman’s Rho correlation) and adult (right: 18 cells from six mice, P = 0.0451, Spearman’s rho correlation) mice. (F) Four-hour heat stress (38°C) selectively induced the c-fos expression in TDNs in DMH (higher magnification inset shown in right). (G) Fraction of c-fos+ TDNs in VZ and HP of DMH and fraction of all c-fos+ and c-fos− neurons in DMH (n = 3 mice). PH, posterior hypothalamus. Scale bars, 100 μm (F) and 20 μm (F, inset). *P < 0.05 and ***P < 0.001.