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Grading scale used to determine immunohistochemical positive pSTAT3 cells in the villus and crypt area
| Grade | Epithelial region | Lamina propria region |
|---|---|---|
| 0 | No staining | No staining |
| 1 | 1‐5 positive cells | 1‐50 positive cells |
| 2 | 5‐10 positive cells | 50‐100 positive cells |
| 3 | >10 positive cells | >100 positive cells |
Notes: The scoring of pSTAT3 expression was applied for the lamina propria and the epithelial regions as previously published. 23
