Non-specific fluorescence turn-on by interaction with biomolecules.

% of max F.I.a	Sq2B2PEG	Sq2B2	Sq2B	SqB
MeOH	100	100	100	100
H2O	13.30	2.067	7.47	28.93
PBS	6.96	2.060	2.098	16.99
BSA	40.075	31.17	38.56	243.50
FBS	18.36	8.20	9.084	127.91
Avidin	6.83	2.31	1.98	21.18
Streptavidin	7.075	2.56	1.51	11.75
Biotin	7.25	2.24	4.56	19.74
DOPC vesicles	21.33	7.81	17.32	91.35

^aF.I. is fluorescence increase. Concentration of probes was fixed to 0.2 μM. Concentrations of biomolecules: BSA (0.1 mg mL⁻¹), FBS (0.1 mg mL⁻¹), avidin (100 nM), streptavidin (100 nM), biotin (100 μM) or DOPC vesicles (20 μM).