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. 2021 May 14;15:680994. doi: 10.3389/fnins.2021.680994

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Copyright © 2021 Andresen, Coreas, Villavisanis and Lauer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The top row shows representative images of the stria vascularis, endolymphatic duct, posterior semicircular canal, superior semicircular canal, and utricular wall in human temporal bones taken at 10× magnification using light microscopy. The stria vascularis is outlined (region of interest) in the top left panel. For the vestibular system the entire image as pictured above was used as the region of interest to measure pigment content. The bottom rows shows the images after color thresholding on ImageJ with the pigmented areas shaded in yellow.