Fig. 1. Acid ceramidase activity monitoring by flow cytometry. AC-catalyzed hydrolysis of the specific AC substrate (RBM5-177) occurs in the lysosome, wherein the reaction product (RBM5-019) accumulates and leads to lysosome staining after the click reaction with a BODIPY-substituted bicyclononyne. Comparative studies with the corresponding canonical counterparts (RBM2-37 and RBM2-31) are illustrative. FC, flow cytometry.
