Fig. 5. DNP ssNMR analysis of U-[13C,15N]–l-Tyr–melanin. (a) 2D 13C–13C SPC5 spectrum showing correlations from the tyrosine ring, DHI, and DHICA units. (b) 2D 13C–15N TEDOR spectra acquired with mixing times of 3.2 ms showing pyrrole correlations show only aromatic contacts and no carboxylated motifs. (c) The aliphatic region shows four distinct Ca–N environments. (d) C4–N correlations from DHICA units were detected.
