Fig. 4. Real-time variations in intracellular GSH levels in response to divalent platinum (PtII) and tetravalent platinum (PtIV) anticancer drugs. (a) Real-time ratiometric imaging of GSH in different timepoints. (b) Real-time average values of GSH concentration detected with 1 (10 μM) in A549 and A549-DDP cells upon cisplatin (10 μM) treatment (A549: n = 77; A549-DDP: n = 86). (c) Average GSH concentration detected with 1 (10 μM) in A549/A549-DDP cells upon cisplatin (10 μM) treatment in different timepoints (A549: n = 77; A549-DDP: n = 86). (d) Chemical structures of cisplatin, oxoplatin and oxclplatin; (e) average values of GSH concentration with 1 (10 μM) in A549 cells upon oxoplatin and oxclplatin (10 μM) treatment at different timepoints (n = 15). Scale bar, 50 μm. error bar represent s.d. *p < 0.05,**; p < 0.01.
