Fig. 2. GrsA/TycB1 affinity determined by MST. GrsA was fluorescently labeled with NT-647-NHS ester (red dye). For MST (microscale thermophoresis) measurements 1 nM of labeled GrsA was incubated with a dilution series of TycB1(WT) as indicated. The experiment was performed in triplicate and error bars represent standard deviations.
