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. 2021 May 11;10:e67914. doi: 10.7554/eLife.67914

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Figure 3. — (A) Exemplary traces showing VGSC currents activated by voltage steps from −80 in 10 mV increments (left), in nucleated patches isolated from Nes^Cre (blue) and Casr^-/- (red) neurons in solutions T_1.1 and T_0.2. The VGSC currents elicited by 10 ms depolarizations to −50 mV (bold) were greater following the switch to T_0.2. (B) Exemplary traces showing VGSC currents activated by voltage steps to −20 mV following a 100 ms conditioning step (left), in the same patches as (A) using solutions T_1.1 and T_0.2. The VGSC currents elicited following conditioning steps to −80 mV (bold) were smaller following the switch to T_0.2. (C) Current-voltage plots of average normalized VGSC currents in nucleated patches from Nes^Cre (n = 8) and Casr^-/- (n = 11) neurons in T_1.1 (filled circles) and T_0.2 (open circles). Currents were normalized using the maximum VGSC current in each recording. (D) Plot of average normalized conductance versus voltage in patches from Nes^Cre neurons for activation (square, n = 8) and inactivation (circle, n = 8) in solutions T_1.1 (filled) and T_0.2 (open). Boltzmannn curves are drawn using average values from individual fits and gray broken lines indicate V_0.5 values for each condition. (E) Plot of average normalized conductance versus voltage in patches from Casr^-/- neurons for activation (square, n = 11) and inactivation (circle, n = 12) in solutions T_1.1 (filled) and T_0.2 (open). Boltzmannn curves are drawn using average values from individual fits and gray broken lines indicate V_0.5 values for each condition. Inset shows plot expanded to emphasize voltage dependence of the window currents. (F and G) represent the plots of D and E expanded to emphasize the voltage dependence of the window currents. (H) Histogram showing V_0.5 for VGSC inactivation in T_1.1 and T_0.2 in patches from Nes^Cre and Casr^-/- neurons. (I) Histogram showing V_0.5 for VGSC activation in T_1.1 and T_0.2 in patches from Nes^Cre and Casr^-/- neurons.