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. 2021 May 10;10:e64045. doi: 10.7554/eLife.64045

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Strain, strain background (Psychrobacter sp.) D2 This study;Zhang Laboratory Wild-type isolate; Available from Zhang lab
Strain, strain background (Psychrobacter sp.) ΔdddX This study;Zhang Laboratory the dddX gene deletion mutant of Psychrobacter sp. D2; Available from Zhang lab
Strain, strain background (Psychrobacter sp.) ΔdddX/pBBR1MCS-dddX This study;Zhang Laboratory ΔdddX containing pBBR1MCS-dddX plasmid; Available from Zhang lab
Strain, strain background (Psychrobacter sp.) ΔdddX/pBBR1MCS This study;Zhang Laboratory ΔdddX containing pBBR1MCS plasmid; Available from Zhang lab
Strain, strain background (Escherichia coli) WM3064 Dehio and Meyer, 1997 Conjugation donor strain
Strain, strain background (Escherichia coli) DH5α Vazyme Biotech company (China) Transformed cells for gene cloning
Strain, strain background (Escherichia coli) BL21(DE3) Vazyme Biotech company (China) Transformed cells for gene expression
Recombinant DNA reagent pK18mobsacB-Ery Wang et al., 2015b Gene knockout vector
Recombinant DNA reagent pK18Ery-dddX This study;Zhang Laboratory pK18mobsacB-Ery containing the homologous arms of the dddX gene of Psychrobacter. sp. D2; Available from Zhang lab
Recombinant DNA reagent pBBR1MCS Kovach et al., 1995 Broad-host-range cloning vector
Recombinant DNA reagent pBBR1MCS-dddX This study;Zhang Laboratory pBBR1MCS containing the dddX gene and its promoter of Psychrobacter. sp. D2; Available from Zhang lab
Recombinant DNA reagent pET-22b-dddX This study;Zhang Laboratory Used for dddX expression; Available from Zhang lab
Commercial assay or kit Pierce BCA Protein Assay Kit Thermo, USA Protein assay
Commercial assay or kit Bacterial genomic DNA isolation kit BioTeke Corporation, China DNA extraction
Commercial assay or kit RNeasy Mini Kit QIAGEN, America RNA extraction
Commercial assay or kit PrimeScript RT reagent Kit Takara, Japan Reverse transcription
Commercial assay or kit Genome sequencing of Psychrobacter sp. D2 Biozeron Biotechnology Co., Ltd, China NCBI: JACDXZ000000000
Commercial assay or kit Transcriptome sequencing of Psychrobacter sp. D2 BGI Tech Solutions Co., Ltd, China NCBI: PRJNA646786
Software, algorithm HKL3000 program Minor et al., 2006 Diffraction data analysis
Software, algorithm CCP4 program Phaser Winn et al., 2011 Diffraction data analysis
Software, algorithm Coot Emsley et al., 2010 Diffraction data analysis
Software, algorithm Phenix Adams et al., 2010 Diffraction data analysis
Software, algorithm PyMOL Schrödinger, LLC http://www.pymol.org/
Software, algorithm MEGA 7 Kumar et al., 2016 Phylogenetic analysis