Strain, strain background (Psychrobacter sp.) |
D2 |
This study;Zhang Laboratory |
|
Wild-type isolate; Available from Zhang lab |
Strain, strain background (Psychrobacter sp.) |
ΔdddX
|
This study;Zhang Laboratory |
|
the dddX gene deletion mutant of Psychrobacter sp. D2; Available from Zhang lab |
Strain, strain background (Psychrobacter sp.) |
ΔdddX/pBBR1MCS-dddX
|
This study;Zhang Laboratory |
|
ΔdddX containing pBBR1MCS-dddX plasmid; Available from Zhang lab |
Strain, strain background (Psychrobacter sp.) |
ΔdddX/pBBR1MCS |
This study;Zhang Laboratory |
|
ΔdddX containing pBBR1MCS plasmid; Available from Zhang lab |
Strain, strain background (Escherichia coli) |
WM3064 |
Dehio and Meyer, 1997
|
|
Conjugation donor strain |
Strain, strain background (Escherichia coli) |
DH5α |
Vazyme Biotech company (China) |
|
Transformed cells for gene cloning |
Strain, strain background (Escherichia coli) |
BL21(DE3) |
Vazyme Biotech company (China) |
|
Transformed cells for gene expression |
Recombinant DNA reagent |
pK18mobsacB-Ery |
Wang et al., 2015b
|
|
Gene knockout vector |
Recombinant DNA reagent |
pK18Ery-dddX
|
This study;Zhang Laboratory |
|
pK18mobsacB-Ery containing the homologous arms of the dddX gene of Psychrobacter. sp. D2; Available from Zhang lab |
Recombinant DNA reagent |
pBBR1MCS |
Kovach et al., 1995
|
|
Broad-host-range cloning vector |
Recombinant DNA reagent |
pBBR1MCS-dddX
|
This study;Zhang Laboratory |
|
pBBR1MCS containing the dddX gene and its promoter of Psychrobacter. sp. D2; Available from Zhang lab |
Recombinant DNA reagent |
pET-22b-dddX
|
This study;Zhang Laboratory |
|
Used for dddX expression; Available from Zhang lab |
Commercial assay or kit |
Pierce BCA Protein Assay Kit |
Thermo, USA |
|
Protein assay |
Commercial assay or kit |
Bacterial genomic DNA isolation kit |
BioTeke Corporation, China |
|
DNA extraction |
Commercial assay or kit |
RNeasy Mini Kit |
QIAGEN, America |
|
RNA extraction |
Commercial assay or kit |
PrimeScript RT reagent Kit |
Takara, Japan |
|
Reverse transcription |
Commercial assay or kit |
Genome sequencing of Psychrobacter sp. D2 |
Biozeron Biotechnology Co., Ltd, China |
NCBI: JACDXZ000000000
|
|
Commercial assay or kit |
Transcriptome sequencing of Psychrobacter sp. D2 |
BGI Tech Solutions Co., Ltd, China |
NCBI: PRJNA646786 |
|
Software, algorithm |
HKL3000 program |
Minor et al., 2006
|
|
Diffraction data analysis |
Software, algorithm |
CCP4 program Phaser |
Winn et al., 2011
|
|
Diffraction data analysis |
Software, algorithm |
Coot |
Emsley et al., 2010
|
|
Diffraction data analysis |
Software, algorithm |
Phenix
|
Adams et al., 2010
|
|
Diffraction data analysis |
Software, algorithm |
PyMOL |
Schrödinger, LLC |
|
http://www.pymol.org/
|
Software, algorithm |
MEGA 7 |
Kumar et al., 2016
|
|
Phylogenetic analysis |