Table 1.
Oligonucleotide sequences used in this study and thermal cycling conditions.
| Gene | Sequence of primers | Thermal cycles | Product size (bp) | Ref |
|---|---|---|---|---|
| mexA | F: CTCGACCCGATCTACGTC | 95°C/5 min; (95°C/1 min, 56°C/30sec, 72°C/45sec) X30; 72°C/5 min | 503 | [8] |
| R: GTCTTCACCTCGACACCC | ||||
|
| ||||
| mexB | F: TGTCGAAGTTTTTCATTGAG | 95°C/5 min; (95°C/1 min, 58°C/45sec, 72°C/45sec) X35; 72°C/5 min | 280 | [8] |
| R: AAGGTCAC GGTGATGGT | ||||
|
| ||||
| oprM | F: GATCCCCGACTACCAGCGCCCCG | 95°C/5 min; (95°C/1 min, 57°C/1 min, 72°C/45sec) X30; 72°C/5 min | 247 | [8] |
| R: ATGCGGTACTGCGCCCGGAAGGC | ||||
|
| ||||
| oprD | F: ATCTACCGCACAAACGATGAG | 95°C/5 min; (95°C/1 min, 59°C/45sec, 72°C/1 min) X30; 72°C/5 min | 156 | [8] |
| R: GCCGAAGCCGATATAATCAAAC | ||||
|
| ||||
| tetA | F: AAGAATCCGCGCGTTCAATCG | 95°C/7 min; (95°C/1 min, 55°C/1 min, 72°C/50sec) X35; 72°C/10 min | 138 | [15] |
| R: GCCCGGCACCGGCATAAT | ||||
|
| ||||
| tetR | F: CCGAATGCGTATGATTCTCC | 95°C/10 min; (95°C/1 min, 57°C/30sec, 72°C/1 min) X30; 72°C/10 min | 84 | [15] |
| R: CGCTTTACTGGCACTTCAGC | ||||
|
| ||||
| emrE | F: CCGCCATGACCAACTATCTC | 95°C/5 min; (95°C/1 min, 58°C/1 min, 72°C/1 min) X30; 72°C/5 min | 249 | [6] |
| R: GCTGGCCGTAGACGAACATC | ||||