Figure 1.

Design of the TALEN for disrupting HLA DR expression on HDF and characterization of HLA DR knockout cells. (a) Schematic of TALEN binding sites within the HLA DR region. The red and underlined nucleotides highlight the anticipated binding sites for the left and right arms of TALEN. (b) Levels of HLA DR genetic disruption determined by the T7E1 assay. The lower bands (arrows) mark the digestion products, indicating TALEN-mediated gene modification. (c) Fluorescent intensity of wild-type and mutant fibroblast cells are different after transfection of the TALEN-encoding plasmids. (d) Alignment of the genomic sequences of wild type and mutant clone at the TALEN recognition site.