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Loss of HLA DR expression on HLA DR knockout fibroblast after genome editing with TALENs. Loss of HLA DR protein expression in HLA DR knockout cells. HLA DR protein expression in wild-type and HLA DR knockout fibroblasts was analyzed by (a) FACS analysis and (b) western blotting. IFN-γ (50 ng/ml or 200 ng/ml) was treated for 5 days on both cell types.
